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Brains were serially sectioned at 40 m using either a cryostat (Leica CM3050S, Leica Biosystems) or a microtome (HM450, Thermo Fisher Scientific) and slices were stored in 0.01% sodium azide in PBS at 4C

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If you hear a change in the hive's buzzing tone or see bees becoming restless and aligning to face you, waft a gentle puff of smoke across the top of the frames to re-mask any potential alarm pheromones